Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR
نویسندگان
چکیده
منابع مشابه
Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR.
The suitability of a 16S rRNA-based mycoplasma group-specific PCR for the detection of mycoplasma contamination in cell cultures was investigated. A total of 104 cell cultures were tested by using microbiological culture, DNA fluorochrome staining, DNA-rRNA hybridization, and PCR techniques. A comparison of the results obtained with these techniques revealed agreement for 95 cell cultures. Disc...
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These antibiotic treatments had a high efficiency of permanent cure: MRA 64%, ciprofloxacin 77%, BM-Cyclin 84%. Resistance to mycoplasma eradication was observed in some cell cultures: BM-Cyclin 5%, ciprofloxacin 14%, MRA 22%. Nearly all resistant contaminants that could be identified belonged to the species M. arginini and M. orale. Detrimental effects of the antibiotics were seen in form of c...
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Both C. pneumoniae and M. pneumoniae are common causes of respiratory tract infection. At present, both are still diagnosed in the laboratory retrospectively by serology. This is despite many publications which indicate that PCR, which is not retrospective, is extremely good at detecting these organisms. We thought that a single PCR test which could detect both organisms simultaneously in a...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1994
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.60.1.149-152.1994